An Appraisal And Evaluation Of Frap Antioxidant Power And Hepatoprotective Activity Of A Herbal Blend Comprising Two Cold Macerated Methanol Extracts Of Litsea Polyantha Linn And Angelica Sinensis In Hepg2 Cells

Abstract

The present study aimed to investigate the antioxidant and hepatoprotective activity of an herbal blend consisting of two medicinal plant extracts viz, methanol leaf extract of Litsea polyantha Linn and Angelica sinensis. The total phenolic contents were assayed for the extracts and for the herbal blend using Folin-Ciocalteu technique. In vitro antioxidant activity was screened and evaluated using the ferric reducing/antioxidant power (FRAP) method. In vitro hepatoprotective activity of the herbal blend was investigated against CCl4-caused hepatic cell injury model in HepG₂ cell lines. In the culture supernatant layer, the hepatic marker enzymes viz., ALT, AST, and LDH were estimated and also estimated the levels of GSH and MDA in lysates of cell. The results demonstrated the significant synergistic activity of the herbal blend against ccl4 induced hepatic injury in HepG₂ cells. The herbal blend also indicated significant antioxidant activity in terms FRAP assay.