An exhaustive investigation of Physicochemical and Antimicrobial activities of Vedi Annabedhi Chendhooram

Abstract

In India, the Siddha system of medicine, predominantly followed by the Tamil people, confines a diverse range of formulations, categorized into Agamarunthu (Internal medicines) and Puramarunthu (External medicines), derived from plant, metal-mineral and animal sources. Especially, the Siddha system places significant emphasis on higher order medicines originating from metals and minerals.Vedi Annabedhi Chendhooram (VABC) is a traditional herbo-mineral formulation utilized for addressing a spectrum of health conditions, such as jaundice, anemia, dysentery, fever, dropsy, ascites and generalized anasarca. To elucidate the Physicochemical and antimicrobial activity of VABC. The Physicochemical analysis of VABC was performed according to Pharmacopoeial Laboratory for Indian Medicine (PLIM) Guideline for standardization and evaluation of Indian medicine (AYUSH systems). Antimicrobial activity of VABC performed by Determination of Minimum inhibitory concentration (MIC) using Resazurin MicrotitreAssay. The results of physicochemical analysis of VABC were done such as Loss on drying at 105^oC was 0.48±0.045%, Total ash was 87.1 ± 2.12, acid insoluble ash was 2.76±0.60%, alcohol soluble extractive was 2.53 ±0.65, water soluble extractive was 5.03 ± 1.18 and it was alkaline in nature with pH of 8.2. The results of antimicrobial assay revealed VABC exhibits notable antimicrobial activity against Klebsiella pneumonoiae and Escherichia coli, demonstrating a substantial effect. Additionally, it exerts a moderate inhibitory effect against Pseudomonas aeruginosa and Staphylococcus aureus. This preliminary study encompassing physicochemical analysis and assessment of the antimicrobial activity of VABC, serves as a crucial foundation for meeting the criteria necessary for subsequent preclinical and clinical research