Isolation, Screening And Morphological Identification Of Fungal L-Methionase From Rotten Fruits And Vegetables

Abstract

Globally, cancer is becoming a more common cause of both mortality and morbidity. L-Methionase is one of the few microbial enzymes with high therapeutic value since it has been reported as an effective anticancer agent against many types of tumor cell lines like breast, lung, colon, kidney, and glioblastoma. Normal cells have a high requirement of amino acids as nutrients, without amino acids, tumor cells fail to function because protein can't be synthesized and tumor cells will die. L-Methionase has important biotechnological applications because of exhibits hydrolytic properties to catalyze α-γ elimination of L-methionine, an essential amino acid to α-ketobutyrate, methanethiol, and ammonia. The catalytic activity of L-Methionase could be used as enzyme supplementation therapy for these diseases. enzyme has been extensively studied by a wide range of organisms, including bacteria, fungi, protozoa, and plants. As a result, of the current study, we obtain 24 fungal isolates from rotten fruits and vegetables. Qualitative and quantitative screening assay test shows the ability of seven isolates to grow with yellow zones surrounding their colonial

growth. MFL 9 (3.45 µmol/min/ml) and MFL24 (2.95 µmol/min/ml) exhibit the highest L-Methionase enzyme activity according to the Nesslerization assay performed for the detection of ammonia.  Therefore, the current study indicates that the enzyme extract from the new isolates has enhanced L-Methionase activity and could be a novel and promising therapy for cancer.