Quantification Of Phytochemicals, Formulation, And Evaluation Of Phyllanthus Niruri Hydrogel For Treating Microbial Infections

Abstract

Phyllanthus Niruri, known for its medicinal properties, was studied for its phytochemical composition, hydrogel formulation, and therapeutic potential against microbial infections. This research aimed to quantify the active components using ethanol and methanol extracts and incorporates these into three different hydrogel formulations, designated F1, F2, and F3. The study assessed the hydrogels for pH, viscosity, extrudability, and spreadability to determine their suitability for medical use. In vitro drug release was examined through first-order kinetic modeling, and the stability of each formulation was tested under varying conditions of temperature, humidity, and light exposure. Results showed significant presence of alkaloids, flavonoids, saponins, terpenoids, and steroids, with variations influenced by the solvent used. F3 emerged as the most effective formulation, offering the quickest drug release and highest ease of application, suitable for acute treatment scenarios. The hydrogels displayed good stability, maintaining their physical and chemical integrity over a six-month period under recommended storage conditions. This study validates the potential of Phyllanthus Niruri hydrogel as a viable alternative in microbial infection treatment, with implications for reducing antibiotic reliance.