Exploring The Effects Of Secretome From Wharton's Jelly Mesenchymal Stem Cells In Zebrafish

Abstract

Cell-free therapy based on conditioned media derived from mesenchymal stem cells has gained attention in the field of protective and regenerative medicine, due to their high content of growth, trophic and protective factors. However, the exact composition and properties of conditioned media can vary greatly depending on multiple parameters, so that the evaluation in vivo of the effects and biosafety of these products is essential. Our recent efforts have been focused on the optimization of a procedure for high-efficient preparation of toxic-free conditioned medium derived from Wharton's jelly mesenchymal stem cells. Moreover, by a multiparametric analysis in zebrafish, we showed that exposure to our conditioned medium preparations triggers antioxidant, anti-apoptotic and pro-regenerative effects. Building on this evidence, here I report detailed morphometric analysis showing that morphological parameters of developing zebrafish embryos were largely unaffected by exposure to conditioned medium, thus confirming the biosafety in vivo of our conditioned medium preparations. This information represents a further step towards future therapeutic application of our conditioned medium preparation.