Green Synthesis Of Manganese Dioxide And Copper Oxide Nanoparticles Using Phaseolus Lunatus Flower Extract And Evaluation Of Their Antimicrobial Activity

Abstract

Manganese dioxide nanoparticles (MnO₂-NPs) and copper oxide nanoparticles (CuO-NPs) with suitable surface chemistry possess various beneficial properties that can be used in numerous biomedical applications, including magnetic resonance imaging contrast enhancement, tissue regeneration, hyperthermia, drug delivery, and cell separation. The study discovered that undiscovered Phaseolus Lunatus flower extract can effectively synthesize manganese dioxide nanoparticles (MnO₂-NPs) and copper oxide nanoparticles (CuO-NPs). The nanoparticles were analyzed by UV-visible spectrophotometer, SEM, EDS, XRD, and FT-IR to determine their properties. The synthesized MnO₂-NPs and CuO-NPs were naturally stabilized, cubic in shape, and sized between 26.98 nm and 65.20 nm. The phytochemicals in the leaf act as reducing agents, aiding in the environmentally friendly synthesis of MnO₂-NPs and CuO-NPs with improved antibacterial properties. The functional groups found on the nanoparticles are primarily -OH and -COOH, as indicated by FT-IR analysis, which makes them hydrophilic. Therefore, no additional functional modifications are required for their uses. The synthesized MnO₂ and CuO were tested for their antibacterial activity against Gram-negative (E. coli and P. aeruginosa) and Gram-positive (S. aureus and B. subtilis). The zone of inhibition measured 17 mm for E. coli and 15 mm for S. aureus. Naturally stabilized Gram-negative nanoparticles with herbal properties can be used in a variety of biological applications.